Brief Reports
Copyright ©2005 Baishideng Publishing Group Co.
World J Gastroenterol. Jan 21, 2005; 11(3): 382-385
Published online Jan 21, 2005. doi: 10.3748/wjg.v11.i3.382
Table 1 Oligonucleotide primers used for cagA , vacA and iceA genotyping.
Region detectedPrimer designationPrimer sequencePCR product (bp)
s1 and s2VA1-F5’ATGGAAATACAACAAACACACC3'259/286
VA1-R5’CTGCTTGAATGCGCCAAACTTTATC3'
s1aSS1-F5’GTCAGCATCACACCGCAAC3'190
s1bSS3-F5’AGCGCCATACCGCAAGAG3'187
s1cS1C-F5’CTYGCTTTAGTRGGGYTA-3'213
m1VA3-F5’GGTCAAAATGCGGTCATGG3'290
VA3-R5’CCATTGGTACCTGTAGAAAC3'
m1Tm1T-F5’GGCCACAATGCAGTCATGG3'290
m1T-R5’CTCTTAGTGCCTAAAGAAACA3'
m2VA4-F5’GGAGCCCCAGGAAACATTG3'352
VA4-R5’CATAACTAGCGCCTTGCAC3'
iceA1iceA1F5’GTGTTTTTAACCAAAGTATC3'247
iceA1R5’CTATAGCCASTYTCTTTGCA3'
iceA2iceA2F5’GTTGGGTATATCACAATTTAT3'229
iceA2R5’TTRCCCTATTTTCTAGTAGGT3'
lcagAlcagAD0085’ATAATGCTAAATTAGACAACTTGAGCGA3'297
lcagAR0085’TTAGAATAATCAACAAACATCACGCCAT3'