CYP2E1-dependent hepatotoxicity and oxidative damage after ethanol administration in human primary hepatocytes

Figure 1 Dose- and time-dependent CYP2E1 activity by ethanal administration. A: Ethanol dose-dependent induction of CYP450 2E1. Hepatocytes were incubated with various concentrations of ethanol (0-100 mmol/L) for 9 h. CYP2E1 enzyme activity was measured as described in Materials and methods. CT: control; ^aP<0.05, ^bP<0.01 vs untreated control cultures. Each point represents the mean±SE of triplicates of five independent experiments; B: Ethanol time-dependent induction of CYP2E1 enzyme activity in human hepatocytes. Hepatocytes were incubated with medium in the presence or absence of 100 mmol/L ethanol for various lengths of time (0-24 h). CYP2E1 enzyme activity was measured. CT: control; ^aP<0.05, ^bP<0.01 vs untreated control cultures. Each point represents the mean±SE of triplicates of five independent experiments.