Role of interleukin 18 in acute lung inflammation induced by gut ischemia reperfusion

Figure 1 Gut ischemia/reperfusion results in increased levels of IL-18 in serum and lung. (A and B) The circulating levels of IL-18 after gut ischemia/reperfusion. Mice were subjected to gut ischemia/reperfusion or sham. Serum IL-18 at the indicated times after reperfusion was collected, part of which was immunoprecipitated with polyclonal rabbit anti-murine IL-18 antibody and analyzed by immunoblotting (A), and another part was assayed with ELISA (B) (n = 5). (C) Gut ischemia/reperfusion results in elevated levels of IL-18 in lung tissue. Immunohistochemistry of lung sections obtained from normal (a, ×100, and b, ×200), 1 h (c) or 3 h (d) I/R model. The positive staining for IL-18 shows as dark brown. Magnification (c and d) ×200.