Antibody to eosinophil cationic protein suppresses dextran sulfate sodium-induced colitis in rats

Figure 4 Detection of ED1 (A) and MIF (B) by Western blot analysis in colonic tissues after 7 d of DDS treatment in normal serum-treated rats (lanes 1-4) and anti-ECP-treated rats (lanes 5-7). The colonic tissue was collected from the lesion area and examined by Western blot analysis as described under Materials and methods. The relative expression levels of ED1 and MIF in the damaged colonic tissue were significantly lower in anti-ECP-treated rats than in normal serum-treated rats. The relative protein expression was quantified by densitometric analysis (n = 7, ^aP<0.05 vs DSS+normal serum).