Liver-specific gene expression in mesenchymal stem cells is induced by liver cells

Figure 4 FACS-pictograms. One example out of three experiments from sorting of GFP+ and GFP- cells of cocultures at wk 2 is shown. A: Viable cells were gated as P1; B: P1 cells were gated in GFP- or GFP+ (P2); C: For highest purification of GFP+ cells, P2 was analyzed and only GFP+ cells were sorted in the sample tube for PCR-analysis (dark gray peak). 80.8% of cells from P2 were GFP+(sorted into the GFP+ test tube) and 19.2% were GFP-.