Liver-specific gene expression in mesenchymal stem cells is induced by liver cells

Table 1 RT-PCR analysis

Primer name	Sequence	PCR conditions	Fragment length	Refer-ence
GAPDH	S: 5’-CCT TCA TTG ACC TCA ACT AC -3’	60 °C; 30×	593 bp	14
	A: 5’-GGA AGG CCA TGC CAG TGA GC-3’
Thy1	S: 5’-CGC TTT ATC AAG GTC CTT ACT C-3’	52 °C; 29×	343 bp	39
	A: 5’-GCG TTT TGA GAT ATT TGA AGG T-3’
CK-18	S: 5’-GGA CCT CAG CAA GAT CAT GGC-3	60 °C; 30×	518 bp
	A: 5’-CCA CGA TCT TAC GGG TAG TTG-3
CK-19	S: 5’-ACC ATG CAG AAC CTG AAC GAT-3’	60 °C; 30×	261 bp	30
	A: 5’-CAC CTC CAG CTC GCC ATT AG-3’
AFP I.	S I: 5’-AAC AGC AGA GTG CTG CAA AC-3	55 °C; 35×	13
	A I: 5’-AGG TTT CGT CCC TCA GAA AG-3’
AFP II. (nested)	S II: 5’-CAC CAT CGA GCT CGC CTA TT-3	60 °C; 30×	619 bp	13
	A II: 5’-TGA TGC AGA GCC TCC TGT TG-3’
Albumin	S: 5’-ATA CAC CCA GAA AGC ACC TC-3’	60 °C; 30×	416 bp	13
	A: 5’-CAC GAA TTG TGC GAA TGT CAC-3’