Expression of adhesion molecules on mature cholangiocytes in canal of Hering and bile ductules in wedge biopsy samples of primary biliary cirrhosis

Figure 4 Localization of ICAM-1 ( A and B) and LFA-1 ( C and D) mRNA at bile ductules in PBC liver tissue by in situ hybridization with CSA. A: ICAM-1 mRNA was expressed in a cytoplasmic pattern on epithelial cells of bile ductile. ( A: ×100 and B: ×300) Arrowhead denotes reaction product; C: LFA-1 mRNA is expressed in a cytoplasmic pattern in lymphocytes around bile ductile. ( C:×100 and D: ×300). Negative controls: E: ICAM-1 sense probe, F: LFA-1 sense probe. All panels: color was developed by DAB chromogen and hematoxylin counterstained. Arrowhead denotes reaction product.