Expression of adhesion molecules on mature cholangiocytes in canal of Hering and bile ductules in wedge biopsy samples of primary biliary cirrhosis

Figure 2 Immunohistochemical distributions of ICAM-1 ( A and C) and LFA-1 ( B and D) in control liver tissue ( A and D) and liver tissue of PHC ( C and D). A: ICAM-1 protein expression on sinusoidal lining cells but no specific immunoreactivity on bile duct epithelial cells in control liver specimens; B: LFA-1 immunoreactivity in portal lymphoid cells, negative in interlobular bile ducts and sparse expression in bile duct epithelial cells; C: Marked ICAM-1 immunoreactivity on the plasma membrane on the luminal side of bile ductules or possibly small blood vessels. Some lymphocytes around the bile ducts were also positive for ICAM-1. Arrowheads denote localization of ICAM-1 (arrows); D: LFA-1 immunoreactivity in lymphocytes around and among damaged bile ducts (arrowheads). Original magnification; ×400, hematoxylin counterstained.