Procedure for preparing peptide-major histocompatibility complex tetramers for direct quantification of antigen-specific cytotoxic T lymphocytes

Figure 6 Flow cytometry of antigen-specific CD8⁺ T cells stained with A2-NLV tetramer (panels E and F) and A2-GIL tetramer (panels G and H) from both HLA- A2 positive (B) and negative (A) donors. Background staining was performed with streptavidin-PE instead of the tetramers (C and D).