Procedure for preparing peptide-major histocompatibility complex tetramers for direct quantification of antigen-specific cytotoxic T lymphocytes

doi:10.3748/wjg.v11.i27.4180

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Jul 21, 2005 (publication date) through Dec 24, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Jul 21, 2005; 11(27): 4180-4187
Published online Jul 21, 2005. doi: 10.3748/wjg.v11.i27.4180

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Figure 5 SDS-PAGE (75 g/L) analyses of A2-NLV and A2-GIL tetramers under non-reducing conditions without boiling. Lane 1: protein MW marker; lane 2: empty; lane 3: A2-NLV monomer (5 μg); lane 4: A2-NLV tetramer (5 μg A2-NLV monomer+8 μg streptavidin-PE); lanes 5 and 8: streptavidin-PE (8 μg); lane 6: A2-GIL monomer (5 μg); lane 7: A2-GIL tetramer (5 μg A2-GIL monomer+8 μg streptavidin-PE). HC: heavy chain.

Citation: He XH, Xu LH, Liu Y. Procedure for preparing peptide-major histocompatibility complex tetramers for direct quantification of antigen-specific cytotoxic T lymphocytes. World J Gastroenterol 2005; 11(27): 4180-4187
URL: https://www.wjgnet.com/1007-9327/full/v11/i27/4180.htm
DOI: https://dx.doi.org/10.3748/wjg.v11.i27.4180