Procedure for preparing peptide-major histocompatibility complex tetramers for direct quantification of antigen-specific cytotoxic T lymphocytes

Figure 2 SDS-PAGE (150 g/L) analyses of recombinant A2-BSP and β₂m proteins expressed in E. coli strain BL21(DE3). Lane 1: BL21 (pET-β₂m) before IPTG induction; lane 2: BL21 (pET-β₂m)+IPTG; lane 3: supernatant of BL21 (pET-β₂m) lysate; lane 4: washed inclusion body of β₂m; lane 5: MW marker; lane 6: BL21 (pET-3c)+IPTG; lane 7: BL21 (pET-A2-BSP) before IPTG induction; lane 8: BL21 (pET-A2-BSP)+IPTG; lane 9: supernatant of BL21 (pET-A2-BSP) lysate; lane 10: washed inclusion body of A2-BSP. HC: heavy chain.