Ephrin-B2 is differentially expressed in the intestinal epithelium in Crohn’s disease and contributes to accelerated epithelial wound healing in vitro

Figure 5 IEC-6 in vitro scratch wound assays indicate an enhanced wound closure activity upon stimulation with EphB1-Fc. IEC-6 cell monolayers were wounded in a standardized manner using a cell scraper, and relative wound closure was monitored after 6 h and 24 h. Subnanomolar doses of EphB1-Fc (0.05 µg/mL~0.33 nmol/L) significantly enhanced the wound closure (^aP < 0.05). Higher doses did not further increase this effect.