Screening for PreS specific binding ligands with a phage displayed peptides library

Figure 1 Diagram of library construction. A: a schematic representation of the steps in the construction of the phagemid encoding the pVIII fusion protein. pCANTAB5E, a pIII displaying vector; pFuse8, the vector harboring gene 8 coding sequence in replace of gene 3; pFuse8-E, the coding sequence for a specific epitope recognized by the 125E11 mAb is inserted into pFuse8; pFuse8-L, the coding sequences for random peptides are inserted into pFuse8; B: Inserted sequences in pFuse8-E and pFuse8-L after translation. SLNPSWD is the specific epitope recognized by the 125E11 mAb. In pFuse8-L, the epitope is replaced with X3CX7CX3.