Hydrogen sulfide protects colon cancer cells from chemopreventative agent β-phenylethyl isothiocyanate induced apoptosis

Figure 3 A: Effects of low molecular weight sulfur compounds on PEITC mediate loss of cell viability; B and C: Inhibition of PEITC mediated loss of cell viability in HCT116 cells following either co-treatment or pre-treatment (24 h) with H₂S or NAC (125 µmol-1 mmol/L) as determined by MTT or LDH leakage. Data are expressed as mean±SD, (n = 8); D: Co-treatment with either H₂S or NAC (125 µmol-1 mmol/L) inhibits PEITC induced caspase-3 activity as determined at 16 h and Data are representative of 3 or more separate experiments conducted on separate occasions. mean±SD, ^bP < 0.01 vs PEITC treated groups.