C-terminal domain of hepatitis C virus core protein is essential for secretion

Figure 2 Isolation of core proteins from cell culture supernatant. Sf9 cells were infected with recombinant baculoviruses expressing HCV core protein. The culture supernatant was collected at d 3 postinfection. Following removal of cell debris and recombinant baculoviruses, the released core proteins were partially purified through a sucrose cushion and were subjected to velocity gradient centrifugation. Twelve fractions were collected and proteins were detected by Western blot analysis using HCV patient sera.