Depletion of CD25+CD4+T cells (Tregs) enhances the HBV-specific CD8+ T cell response primed by DNA immunization

Figure 4 Functional avidity of S28-39-specific CD8⁺ T cells. The functional avidities of the HBV S28-39-specific CD8⁺ T cells were compared in a peptide dose titration experiment and the ability to secrete IFN-γ upon stimulation with S28-39 peptides was determined. PBMCs from six mice were pooled and incubated for 6 h with indicated concentrations of S28-39 peptide and stained for CD8 and IFN-γ. The data show percentages of IFN-γ producing cells in the CD8⁺ cell population.