Optimization of competitively differentiated polymerase chain reaction in detection of HBV basal core promoter mutation

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Jun 21, 2005 (publication date) through Jul 28, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Jun 21, 2005; 11(23): 3614-3618
Published online Jun 21, 2005. doi: 10.3748/wjg.v11.i23.3614

Table 1 Primers and probe.

Denomination	Sequences
PCSc	5’-CCCGAATTCCACCGTGAACGCCCATCAG-3’
PCAc	5’-CCCAAGCTTGCAGTATGGTGAGGTGAGCAATG-3’
BCP-c1	5’-CGGAATTCGAGGAGCTGGGGGAGGAGATTAGGTTAATGATC-3’
BCP-c2	5’-AGACTGCAGAGGATAGAATCTAGCAGGC-3’
BCP1-W	5’-FITC-CGTCCGTAGTGCCGAGGGAGGAGATTAGGTTAAAGG-3’
BCP1-M	5’-DIG -CGCTGAGATGCGACTGGGAGGAGATTAGGTTAATGA-3’
PCA	5’-CTCGCTGCACACTCTAAGGCTTCTCGATACAG-3’
PCP	5’-BIO-GACGGAAGGAAAGAAGTCAGAAGGCAAAA-3’

The underlined nucleotides were loci of restriction endonucleases. The italic characters had no relationship with HBV genome.

Citation: Peng XM, Gu L, Chen XJ, Li JG, Huang YS, Gao ZL. Optimization of competitively differentiated polymerase chain reaction in detection of HBV basal core promoter mutation. World J Gastroenterol 2005; 11(23): 3614-3618
URL: https://www.wjgnet.com/1007-9327/full/v11/i23/3614.htm
DOI: https://dx.doi.org/10.3748/wjg.v11.i23.3614