Activation of JAK-STAT pathway is required for platelet-derived growth factor-induced proliferation of pancreatic stellate cells

Figure 4 Ethanol and acetaldehyde decreased basal activation of JAK2 and STAT3. PSCs were treated with ethanol (at 50 mmol/L, panel A) or acetaldehyde (at 200 mmol/L, panel B) for the indicated time, or with PDGF-BB (at 25 ng/mL) for 5 min. Total cell lysates (approximately 100 μg) were prepared, and separated by 100 g/L SDS-polyacrylamide gel electrophoresis. The activation of JAK2, STAT1, and STAT3 was examined by Western blotting using anti-phosphospecific antibodies. The level of GAPDH was also determined.