Activation of JAK-STAT pathway is required for platelet-derived growth factor-induced proliferation of pancreatic stellate cells

Figure 2 PDGF activated STAT1 and STAT3. A: PSCs were treated with PDGF-BB (at 25 ng/mL, lane 2) in serum-free medium for 15 min. Nuclear extracts were prepared and subjected to electrophoretic mobility shift assay using STAT consensus oligonucleotide probe m67. Arrow denotes specific inducible complex competitive with cold double-stranded oligonucleotide probe (lane 3). For super shift assays, nuclear extracts were incubated with antibodies against STAT1 (lane 4) or STAT3 (lane 5) before incubation with the radiolabeled probe. *: super shifts. Lane 1: control (serum-free medium only); B: PSCs were treated with PDGF-BB (at 25 ng/mL) for the indicated time. Total cell lysates (approximately 100 μg) were prepared, and separated by 100 g/L SDS-polyacrylamide gel electrophoresis. The activation of STAT1 and STAT3 was examined by Western blotting using anti-phosphospecific antibodies. The levels of total STAT1 and STAT3 were also determined.