Activation of JAK-STAT pathway is required for platelet-derived growth factor-induced proliferation of pancreatic stellate cells

Figure 1 Activated PSCs expressed PDGF β-receptor. A: Serum-starved, culture-activated PSCs were grown directly on slides. Immunostaining for PDGF β-receptor was performed using a streptavidin-biotin-peroxidase complex detection kit. Original magnification: ×20 objective; B: Serum-starved PSCs were treated with PDGF-BB (at 25 ng/mL) for the indicated time. Total cell lysates were prepared, and separated by 70 g/L SDS-polyacrylamide gel electrophoresis. The phosphorylation of PDGF β-receptor was examined by Western blotting.