Different cytokine response of primary colonic epithelial cells to commensal bacteria

Figure 4 Profile of cytokines secreted by CEC in response to commensal bacteria. CECs were cultured for 4 h in complete medium alone (M) or in medium containing Bacteroides ovatus (Bo), E. coli (Ec) or Lactobacillus rhamnosus (Lr) after which conditioned medium was assayed for the presence of IL-6, TNF-α, IL-1β and KC. The amount of cytokine present was determined by reference to a standard curve generated using known amounts of recombinant protein. The limit of detection of each assay was ~5 pg/mL. The results shown were obtained by combining the data sets from a minimum of three independent experiments. Error bars designate 95% confidence limits.