Different cytokine response of primary colonic epithelial cells to commensal bacteria

Figure 3 Changes in expression of cytokine genes in CEC in response to commensal bacteria. CECs were cultured for 4 h in complete medium alone (M) or in medium containing Bacteroides ovatus (Bo), E. coli (Ec) or Lactobacillus rhamnosus (Lr) after which RNA was extracted from CECs and processed for RT-PCR analysis using primers for β-actin and genes encoding cytokines and the anti-microbial peptide, β-defensin3 as described in the Materials and methods section. The results shown are typical of those obtained from a total of six independent experiments.