Green tea polyphenol epigallocatechin-3-gallate blocks PDGF-induced proliferation and migration of rat pancreatic stellate cells

Figure 3 EGCG inhibited PDGF-induced proliferation and migration. A: Serum-starved PSCs were left untreated (“Cont”) or treated with PDGF-BB (at 25 μg/L) in the presence or absence of EGCG at the indicated concentrations (μmol/L). After 24-h incubation, DNA synthesis was assessed by BrdU incorporation enzyme-linked immunosorbent assay. Data are shown as mean±SD (% of the control, n = 6). ^bP<0.01 vs PDGF only; B: cell migration was assessed using modified Boyden chambers with 8-μm pore filters. Serum-starved PSCs were left untreated (“Cont”) or were treated with PDGF-BB (at 25 μg/L) in the lower chamber in the absence or presence of EGCG at the indicated concentrations (μmol/L). After 24-h incubation with PDGF, the cells migrated to the underside of the filter were stained, and counted. Data are shown as mean±SD (% of the control, n = 6). ^bP<0.01 vs PDGF only.