Helicobacter pylori promote gastric cancer cells invasion through a NF-kB and COX-2-mediated pathway

Figure 3 Effect of COX-2 or NF-κB inhibitor on COX-2, MMP-9, or VEGF protein level in MKN-45 cells. A: Cells were cultured in the presence or absence of H pylori and NS398 for 24 h, and cellular protein was extracted and subjected to Western blot analysis; B: MKN-45 cells were treated with or without PDTC and in the presence or absence of H pylori for 24 h. The experiments were repeated on at least three occasions, and the results were identical to these presented here. All blots were stripped and probed with β-actin to check for equal protein loading.