Nerve-pathways of acupoint Fengch’ih in rat by anterograde transport of HRP

Figure 1 The distribution of HRP-positive neurons of cervical dorsal root ganglia, the anterior horn cervical spinal cord, the ventromedial division of the facial nucleus. A: Photomicrograph of the longitudinal sections of the second cervical dorsal root ganglion showed an intense and homogeneous distribution of HRP reaction product within the cell bodies and their processes. The labeled neurons were green and their processes arranged as pine needle stained with Gu YM’s technique. ×400. B: Photomicrograph of horizontal sections of second cervical spinal cord. The labeled neurons were located in the anterior horn. The dark green cells were the HRP-positive neurons and the red cells were the negative neurons stained with Gu YM’s technique and counterstained by neutral red. ×40. C: Photomicrograph of the facial nucleus showed the labeled neurons in the ventromedial division nucleus.The labeled neurons were dark green and the negative neurons were red stained with Gu YM’s technique and counterstained by neutral red. ×100.