Expression and purification of the complete PreS region of hepatitis B Virus

doi:10.3748/wjg.v11.i20.3060

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May 28, 2005 (publication date) through Feb 2, 2025

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Viral Hepatitis

World J Gastroenterol. May 28, 2005; 11(20): 3060-3064
Published online May 28, 2005. doi: 10.3748/wjg.v11.i20.3060

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Figure 3 Purification of thio-PreS. A: Fractions of thio-PreS eluted with increasing imidazole concentrations listed at the bottom of each lane. The triangle indicates the position of thio-PreS; B: Further purification with a freeze-thaw treatment. Lanes 1 and 2: supernatants of the bacteria lysate before or after IPTG induction, respectively; lanes 3 and 4: supernatant and precipitate of the affinity purified thio-PreS sample after a freeze-thaw treatment. Molecular weight maker is denoted as M.

Citation: Deng Q, Kong YY, Xie YH, Wang Y. Expression and purification of the complete PreS region of hepatitis B Virus. World J Gastroenterol 2005; 11(20): 3060-3064
URL: https://www.wjgnet.com/1007-9327/full/v11/i20/3060.htm
DOI: https://dx.doi.org/10.3748/wjg.v11.i20.3060