Molecular mechanisms of denbinobin-induced anti-tumorigenesis effect in colon cancer cells

Figure 3 Effect of denbinobin on caspases, Bid protein levels or Cyto c and AIF translocation from mitochondria to cytosol. The whole cell proteins (A) or cytosolic proteins for Cyto c, AIF (B) were extracted from the cultured COLO 205 cells, which had been grown in 10% FCS and incubated for the indicated times with 0.1% DMSO or 20 μmol/L denbinobin in 0.1% DMSO. After electrophoresis, proteins were transferred onto Immobilon-P membranes, and then probed with proper dilutions of specific antibodies. Membranes were also probed with anti-GAPDH antibody to correct for difference in protein loading. Denbinobin time-dependently induced the activation of caspases and Bid (A), translocation of Cyto c and AIF (B).