Polymerase chain reaction-single strand conformational polymorphism analysis of rearranged during transfection proto-oncogene in Chinese familial hirschsprung’s disease

Figure 2 Sequence analysis of RET gene in patients with aberrant SSCP patterns. The arrows indicate the position of mutation. A: A G heterozygous insertion at nucleotide 18 974 in exon 13, resulted in a frameshift mutation; B: A T to G transition at codon 745 in exon 13 was exchanged from CTT to CTG, resulting in a silent mutation; C: An A to G transition at codon 756 in exon 13 resulted in a K→E missense mutation; D: A G to A substitution at codon 667 in exon 11 resulted in a G→S missense mutation; E: A G to A substitution at codon 916 in exon 15 resulted in a silent mutation.