Expression of sialyl Lewisa relates to poor prognosis in cholangiocarcinoma

Figure 3 Adhesion of CCA cells to epithelial cells of the human umbilical vein endothelial cells (HUVEC). KKU-M213 or KKU-100 cells were incubated with non-activated or IL-1β activated HUVECs for 45 min at 37 °C. The basal adhesion levels of KKU-100 and KKU-M213 to non-activated HUVECs were not statistically different, whereas the adhesion levels to activated HUVECs were significantly different (P<0.001). A significant difference occurred between the levels of KKU-100 and KKU-M213 adhesion to non-activated and IL-1β-activated HUVECs (P<0.001). The adhesion level of KKU-M213 significantly declined (P<0.001), when anti-sLe^a or anti E-selectin was added to KKU-M213 or IL-1β-activated HUVECs before the adhesion assay. The results were expressed as mean±SD of triplicate samples of a representative experiment.