Construction of recombinant eukaryotic expression plasmid containing murine CD40 ligand gene and its expression in H22 cells

doi:10.3748/wjg.v11.i2.182

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Jan 14, 2005 (publication date) through Jan 23, 2025

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Liver Cancer

World J Gastroenterol. Jan 14, 2005; 11(2): 182-186
Published online Jan 14, 2005. doi: 10.3748/wjg.v11.i2.182

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Figure 1 Electrophoresis of recombinant plasmid pcDNA3. 1⁺-mCD40L. Lanes 1 and 5: DNA molecular weight marker; lane 2: The RT-PCR product of mCD40L; lane 3: pcDNA3.1⁺-mCD40L digested with NheI and EcoRI, pcDNA3.1⁺ in the upper, full-length mCD40L-cDNA in the lower; and lane 4: pcDNA3.1⁺-mCD40L not digested with NheI and EcoRI.

Citation: Jiang YF, He Y, Gong GZ, Chen J, Yang CY, Xu Y. Construction of recombinant eukaryotic expression plasmid containing murine CD40 ligand gene and its expression in H22 cells. World J Gastroenterol 2005; 11(2): 182-186
URL: https://www.wjgnet.com/1007-9327/full/v11/i2/182.htm
DOI: https://dx.doi.org/10.3748/wjg.v11.i2.182