Cytotoxic effect of a non-peptidic small molecular inhibitor of the p53-HDM2 interaction on tumor cells

Figure 2 Expression of HDM2, p53 and p21 proteins in HT1080 cells (A) and KYSE510 cells (B) induced by syl-155. The HT1080 cells were harvested at the indicated time after treatment with 10 μg/mL syl-155, and then protein was extracted for Western blot. HDM2, p53, p21 and β-actin proteins were detected using mouse monoclonal antibodies DO-1, D-12, DO-1, F-5 and anti-β-actin antibody as primary antibodies, respectively.