Nestin-positive progenitor cells isolated from human fetal pancreas have phenotypic markers identical to mesenchymal stem cells

Figure 1 The culture (A-C) and immunofluorescence staining (D-F) of nestin-positive cells isolated from human fetal pancreas. A: The free-floating ICCs isolated from fetal human pancreas; B: After pipetted out and cultured in a new dish, the ICCs attached to the bottom and a monolayer of epithelium-like cells spread out; C: The epithelium-like cells making confluent sheet, the ICCs disappeared structurally (×100); D: Negative control using mouse IgG to substitute the primary antibody; E: Nestin expressed in some of the epithelium-like cells spreading out from ICCs. F: Expression of nestin in most of the epithelium-like cells passaged 10 times (×200).