Enhancement of humoral immune responses to HBsAg by heat shock protein gp96 and its N-terminal fragment in mice

Figure 1 Gp96 expressed in E. coli and purification from normal murine livers. A and B: Elutions of gp96 from POROS 20QE column with 300-800 mmol/L NaCl were detected by SDS-PAGE. The gp96 expressed in E.coli. A: or purified from mice livers; B: were collected as 1 mL fractions during the purification on POROS 20QE column and a total of six individual fractions (lanes 1-6) were run on the SDS-PAGE gel with Coomassie blue staining afterwards; C: Gel-filtration analysis of gp96 purified from mice livers. The protein samples are run on Superdex G200 column; D: Proteins collected from the peak of gel filtration were run on 10% SDS-PAGE and Western blot are shown on the right.