Genetic association of cytokines polymorphisms with autoimmune hepatitis and primary biliary cirrhosis in the Chinese

Table 2 PCR primer pairs for genotyping in cytokines gene.

Gene	Position	Primers	Product size (bp)
IL-1B	+3953	5'-GTTGTCATCAGACTTTGACC-3’	249
		5'-TTCAGTTCATATGCACCAGA-3’
IL-1RN	exon2	5'-GCCCCTCAGCAACACTCCTAT-3’
		5'-CATCTTCCTGGTCTGCAGG-3’	1
		5'-CATCTTCCTGGTCTGCAGC-3’
IL-6	-174G/C	5'-CCCTAGTTGTGTCTTGCC-3’	190
		5'-CCCTAGTTGTGTCTTGCG-3’	190
		5'-GAGCTTCTCTTTCGTTCC-3’
IL-10	-1082G/A	5'-TCTTACCTATCCCTACTTCC-3	139
		5'-CTCGCTGCAACCCAACTGGC-3’
	-819T/C	5'-AACTGAGGCACAGAGATG-3	402
		5'-AACTGAGGCACAGAGATA-3	402
		5'-AGCAACACTCCTCGCCGCAAC-3’
	592A/C	5'-GGTGAGCACTACCTGACTAGC-3	412
		5'-CCTAGGTCACAGTGACGTGG-3’
HLA-DRB1	5c	5'- TGC CAA GTG GAG CAC CCA A-3	7962
	3c	5'-GCA TCT TGC TCT GTG CAG AT-3’

¹Variable numbers of an 86 bp tandem repeat sequence.

²The inter-reference of allele-specific PCR.