Increased activity of Pgp multidrug transporter in patients with Helicobacter pylori infection

Table 1 Effect of sample storage.

H pylori positivepatients	D1			D2
	Rh123 MF	Rh123+CsA MF	RMF	Rh123 MF	Rh123+CsA MF	RMF
1	73.14	124.58	1.703	25.00	44.76	1.790
2	75.25	17.86	0.237	27.83	4.28	0.157
3	5.47	20.85	3.404	17.25	41.72	2.419
4	83.40	113.79	1.364	43.03	61.20	1.423
5	83.40	113.79	1.364	90.44	172.18	1.920
6	141.21	68.47	0.485	72.91	80.63	1.106
7	72.31	223.19	3.087	46.72	223.44	4.783
8	262.30	220.78	0.842	308.4	326.90	1.06
9	135.53	93.90	0.693	36.71	255.95	6.972
10	170.21	225.35	1.324	220.95	223.71	1.012
11	115.20	165.20	1.434	37.88	94.26	2.288
12	11.30	25.10	2.221	25.76	134.78	5.332
RMF (mean±SD)	1.427±1.034			2.522±2.067

D 1 = fresh cells, several hours of sampling; d 2 = cells stored overnight at room temperature; Rh123 = rhodamine; CyA = cyclosporine A; MF = mean fluorescence; RMF = ratio of mean fluorescence.