Involvement of fatty acid-CoA ligase 4 in hepatocellular carcinoma growth: Roles of cyclic AMP and p38 mitogen-activated protein kinase

Figure 2 Growth inhibition of Hep3B cells by FACL4 knockdown (A) siRNA expression construction and predicated siRNA transcripts. (B) Colony formation assay. ^aP<0.05 vs mock or empty. pcDNA/FL, sense expression plasmid; pcDNA/antiFL, FACL4 antisense expression plasmid; pKd/non, non-specific siRNA expression plasmid; pKd/FL, FACL4 siRNA expression plasmid. (C-E) Cells transfected with pKd/non or pKd/FL2 plasmid and selected neomycin-resistant clones.