Effect of norcantharidin on proliferation and invasion of human gallbladder carcinoma GBC-SD cells

Figure 4 The positive expression occurred in cell nucleoli, with brown or yellow dye, of Ki-67 protein of GBC-SD cells (immunohistochemistry SABC method, ×100). A: The brown dye of Ki-67 was shown positively in most cells of the control group. B: In the experiment group with treatment of IC₅₀ NCTD for 48 h, the positive cells of Ki-67 expression decreased significantly and the dye in cell nucleoli became light and shallow.