Heterogeneity in predisposition of hepatic cells to be induced into pancreatic endocrine cells by PDX-1

Figure 1 Expression of PDX-1 transgene and other islet-enriched genes in PDX-1⁺HepG₂ cell line. RT-PCR analysis was performed to detect the expression of several islet differentiation markers (A) and islet-specific transcriptional factors (B). GAPDH mRNA was amplified as an internal control. Western blot analysis was performed to detect the expression of PDX-1 using anti-PDX-1 antibody on total protein lysis (C). Lane 1: 24-wk human fetal pancreas (positive control); Lane 2: wild type HepG₂ cells (negative control); Lane 3: PDX-1⁺HepG₂ cells. Data are representative of at least three independent experiments.