Expression of Helicobacter pylori AlpA protein and its immunogenicity

doi:10.3748/wjg.v11.i15.2260

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Apr 21, 2005 (publication date) through Nov 5, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Helicobacter Pylori

World J Gastroenterol. Apr 21, 2005; 11(15): 2260-2263
Published online Apr 21, 2005. doi: 10.3748/wjg.v11.i15.2260

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Figure 3 SDS-PAGE analysis of 100 g/L fusion protein expressed in BL21(DE3). Lane 1: Molecular weight marker (20, 30, 43, 67, 94)×10³; lane 2: BL21 (pET-AlpA) cells before induction; lane 3: BL21 (pET-AlpA) cells in periplasm protein after 3-h induction with IPTG; lane 4: BL21 (pET-AlpA) cells after 3-h induction with IPTG; lane 5: BL21 (pET-AlpA) cells after 5-h induction with IPTG; lane 6: sonicated supernatant of BL21 (pET-AlpA) cells after 3-h induction with IPTG; lane 7: inclusion body of BL21 (pET-AlpA) cells after 3-h induction with IPTG; lane 8: control strain BL21 (pET) before induction; lane 9: control strain BL21 (pET) after 3-h induction with IPTG.

Citation: Xue J, Bai Y, Chen Y, Wang JD, Zhang ZS, Zhang YL, Zhou DY. Expression of Helicobacter pylori AlpA protein and its immunogenicity. World J Gastroenterol 2005; 11(15): 2260-2263
URL: https://www.wjgnet.com/1007-9327/full/v11/i15/2260.htm
DOI: https://dx.doi.org/10.3748/wjg.v11.i15.2260