Differential gene expression during capillary morphogenesis in a microcarrier-based three-dimensional in vitro model of angiogenesis with focus on chemokines and chemokine receptors

Table 1 Primer sequences used for RT-PCR.

Genes	Primer (upstream)	Primer (downstream)	Length (bp) of PCR product	Annealing(temperature)
CYR61	5’-GAATGGAGCCTCGCATCCGATA-3’	5’-GTGGCTGCATTAGTGTCCATCC-3’	703	60
CD31	5’-ATGTGGCTTGGAGTCCTGCTGA-3’	5’-GTGTAGTTGCCACTGTGCTCCA-3’	878	60
RANTES	5’-GGATGGAGAGTCCTTGAACCTG-3’	5’-TTGAGACGGAGTCTCGCTCTGT-3’	441	60
PRSS3	5’-AGCAGCTCACTGCTACAAGACC-3’	5’-AGACCTTGGTGTAGACTCCAGG-3’	518	60
PTGS2	5’-CGGTGAAACTCTGGCTAGACAG-3’	5’-TTGGCTTCCAGTAGGCAGGAGA-3’	941	60
EBI3	5’-CCTTCATTGCCACGTACAGGCT-3’	5’-AGTGACTGGGATTACAGGTGCG-3’	845	60
ANXA1	5’-GTGAAGTCATCCAAAGGTGGTCC-3’	5’-CCTTATGGCGAGTTCCAACACC-3’	811	60
EGR1	5’-AGTGCCATCCAACGACAGCAGT-3’	5’-AGGACTTGGCTCTGAGAACCTC-3’	1 183	60
EDN1	5’-GCTGTTTGTGGCTTGCCAAGGA-3’	5’-GACCTTCGTCAGAAACTCCACC-3’	956	60
ADAM15	5’-TTCCAGCACCTGCTAAACCGCA-3’	5’-CAACTGAGCAGAGACAGGCTGT-3’	708	60
Rac1	5’-TGGTGGGAGACGGAGCTGTA-3’	5’-GGATCGCTTCGTCAAACACTG-3’	791	60
TIMP-1	5’-CACCAGAGAACCCACCATG-3’	5’-GCAGGCTTCAGCTTCCACTC-3’	669	57
TIMP-2	5’-TTTGCAATGCAGATGTAGTG-3’	5’-TCGAGAAACTCCTGCTTGG-3’	534	56
GAPDH	5’-GCCAAAAGGGTCATCATCTC-3’	5’-GTAGAGGCAGGGATGATGTTC-3’	286	60