Identification of the immunogenic domains in HBsAg preS1 region using overlapping preS1 fragment fusion proteins

Figure 5 Identification of the precise immunogenic domains in humans by competitive ELISA. The coated antigens were the seven fusion proteins containing different preS1 fragments: preS1 (21-47) (A), preS1 (34-59) (B), preS1 (48-70) (C), preS1 (60-85) (D), preS1 (71-94) (E), preS1 (86-109) (F) and preS1 (95-119) (G), respectively. The mixture of sera samples from acute hepatitis B patients was previously incubated with the coated antigens or the overlapping antigen(s) at a dose of 2 µg, and the samples not treated with antigen(s) were used as controls.