Identification of the immunogenic domains in HBsAg preS1 region using overlapping preS1 fragment fusion proteins

Figure 4 Detection of antigenicity (A₄₅₀) of the eight different preS1 fragments overlapped in preS1 (21-119) region against hepatitis B patients’ serum samples with different infection types by indirect ELISA. ¹The antigenicity of the fragments, preS1 (21-47), preS1 (48-70), preS1 (86-109), preS1 (95-119), and especially preS1 (34-59), decreased significantly in chronic-phase sera (chronic hepatitis B and HCC with hepatitis B samples) in comparison with acute-phase sera (P<0.05). However, 2the antigenicity of the fragments, preS1 (60-85) and preS1 (71-94), in the middle part showed less diversity (P>0.05). Each column represents the mean A value±SE.