Identification of the immunogenic domains in HBsAg preS1 region using overlapping preS1 fragment fusion proteins

Figure 1 Expression, purification, and Western blot analysis of eight GST fusion proteins containing overlapping preS1 fragments in E. coli BL21 (gold). A and B: Cell lysates and purified fusion proteins subjected to 12.5% SDS-PAGE and stained with Coomassie brilliant blue. C: Protein bands on the duplicate gel of cell lysates electroblotted onto a nitrocellulose membrane for Western blot analysis with mice antisera. Lane M: Molecular weight standard. Lanes 1-8: The samples containing preS1 (21-119), preS1 (21-47), preS1 (34-59), preS1 (48-70), preS1 (60-85), preS1 (71-94), preS1 (86-109) and preS1 (95-119) in a sequent order.