Controlled and reversible induction of differentiation and activation of adult human hepatocytes by a biphasic culture technique

Figure 9 Western blot analysis of milieu dependent shift of MET phosphorylation (MET_pho). Environmental switch has been carried out on d 5 and 10. Left lanes indicate HC cultivation within a 3D collagen matrix (sandwich), right lanes confer to a 2D culture system (collagen matrix). Incubation code A indicates initial 5-d incubation in DM, followed by 5-d incubation in AM and subsequent incubation in DM. Incubation code B is related to initial incubation in AM, followed by incubation in DM and subsequent incubation in AM. The blots are representative of three separate experiments.