Controlled and reversible induction of differentiation and activation of adult human hepatocytes by a biphasic culture technique

Figure 8 Dynamics of EGFR distribution depending on modulation of the culture environment. A: Receptor localization along the plasma membrane after switching the extracellular milieu from de-differentiation (activation) to differentiation inducing conditions (d 10); B: The reverse order (DM-AM) evoked a distinct receptor translocation into the cytoplasm (d 10); C: Receptor re-localization onto the cell surface was induced again after a further 10-d incubation in DM (d 20). Confocal microscopy, ×100/1.3 oil immersion objective. Each figure is representative for three separate experiments.