Controlled and reversible induction of differentiation and activation of adult human hepatocytes by a biphasic culture technique

Figure 7 Western blot analysis of CK 18 protein content. HC were incubated alternately in AM or DM. Medium was changed on d 5 and 10. Code A indicates initial 5-d incubation in DM, followed by 5-d incubation in AM and subsequent incubation in DM. Code B is related to initial incubation in AM, followed by incubation in DM and, subsequently, in AM. β-actin served as the internal control. The blot is representative for three separate experiments.