Controlled and reversible induction of differentiation and activation of adult human hepatocytes by a biphasic culture technique

Figure 6 CK 18 and 19 expression, several time points after cell plating. A: Kinetic part I is related to initial 5-d incubation in DM, followed by 5-d incubation in AM and subsequent incubation in DM; B: Kinetic part II is related to the incubation order AM-DM-AM. HC were seeded on a 2D collagen matrix and incubated alternately in AM or DM. CK level was detected by flow cytometry and expressed as RFU. mean±SD (n = 6); ^aP<0.05 between groups.