Controlled and reversible induction of differentiation and activation of adult human hepatocytes by a biphasic culture technique

Figure 5 Western blot analysis of MET protein content. HC were incubated alternately in AM or DM. Medium was changed on days 5 and 10. Incubation code A indicates initial 5-d incubation in DM, followed by 5-d incubation in AM and subsequent incubation in DM. Incubation code B is related to initial incubation in AM, followed by incubation in DM and, subsequently, in AM. A431 cells served as positive controls. The blots are representative for three separate experiments.