Controlled and reversible induction of differentiation and activation of adult human hepatocytes by a biphasic culture technique

Figure 4 Dynamics of growth factor receptor expression. A: MET kinetics; B: EGFR kinetics. HC were plated on a 2D collagen matrix and incubated alternately in AM or DM. Code A (2D start_diff) is related to initial 5-d incubation in DM, followed by 5-d incubation in AM and subsequent incubation in DM. Code B (2D start_activ) concerns the incubation order AM-DM-AM. The receptor level was detected by flow cytometry and expressed as RFU. mean±SD (n = 6), ^aP<0.05 between groups.