Adenosine 5'triphosphate transport and accumulation during the cold preservation of rat hepatocytes in University of Wisconsin solution

Figure 1 A: Effect of the addition of ATP to the UW solution on intracellular ATP content in hepatocytes. Cells were cold stored (0 °C) up to 72 h in UW solution containing ATP: 5 mmol/L (b-group), 10 mmol/L (c-group) and without ATP (a-group). ATP was determined at time 0 and 72 h. Data are given as the mean±SD for hepatocytes prepared from three rats for each group. The asterisk indicates that after 72 h of cold storage, the ATP content of hepatocytes from groups a and b is statistically different from c-group at ^aP<0.05; B: ATP content of freshly isolated and cold-stored hepatocytes during rewarming. Cells from a-, b- and c-groups were cold preserved (0 °C, 72 h); they were later resuspended and incubated at 37 °C for 120 min in KHR. ATP was determined at time 0 and 120 min. Data are expressed as the mean±SD for three separate preparations. At time 0, the asterisk indicated that groups a and b are different from groups c and controls (^aP<0.05); at 120 min, the asterisk indicated that groups a and b are different from groups c and controls (^aP<0.05).